Glycosides and their derivatives are known for their important biological properties. Phenolic glycosides, such as salicin, belong to most studied glycosides due to their therapeutic effects . Many compounds from this group have been shown to be particularly effective in treatment of pulmonary diseases, arthritis, anti-inflammatory, analgetic and antioxidant activities [2,3]. In order to improve biological activity and efficiency, much effort was exerted to prepare structures possessing salicin or salicin-based moiety combined with heterocyclic arrays. As these structures are rather complex, one can expect that salicin-type glycoconjugates exhibit a multifaceted conformational equilibrium when dissolved in various solvents. Presented analysis deals with 3D structure in solution of new glycoconjugate constituted by two glucopyranoses (Glc) linked via quinazolinone-like structure (Fig. 1) using NMR and DFT calculations.