Topological knots naturally occur on DNA due to conformational moves  or activity of molecular machines . Around half of knots are chiral including the simplest and most abundant trefoil knot. In some bacteria, occurrence of chiral knots is preferred over the achiral ones  and in some one form of chiral knots is more abundant . Experimentally, it was successful to separate chiral knots through 2D-gel electrophoresis, where their structure was altered by negative supercoiling . In general, we know that chirality plays an important role in biological systems, yet there are still mysteries to uncover, especially regarding interaction of chiral molecules towards various environments. In this work, we observe differences between right- and left-handed forms of trefoil knots and their interplay with various confinements (cylinder, toroid, helix) through molecular dynamics simulations in ESPResSo. Simulations in cylinder provided general information about behaviour of knots in confined system, their size and shape properties, which we divided into two categories – geometrical properties independent of chirality and topological parameters influenced by handedness of the knot. Toroid confinement represents a system curved in space in one dimension. Such curving, however, was not enough to enhance distinguishability of chiral knots and they had similar topological properties as in cylinder. Right-handed helix, on the other hand, strongly influenced knot topology and induced symmetry breaking for chiral knots. Right-handed knot was delocalized and compressed at its centre, while the left-handed knot expanded its main loop towards confinement wall, making it more localized, spherical and better fitting in the helix. Different topological behaviour of chiral knots as well as introduced asymmetry visible on radius of curvature indicates that helical confinement is suitable for distinguishing and direct separation of right- and left-handed forms of knots.