Antioxidant, antibacterial and anti-biofilm activity of new unnatural gallotannins

Antioxidant, antibacterial and anti-biofilm activity of new unnatural gallotannins

Overall rating

Scientific work
92%
Design
84%
Discussion interaction
100%
UserScientific workDesignDiscussion interaction
Bc. Daniela Máčalová100%100%100%
Mrs. Malgorzata Ziarno100%100%-
MUDr. Natália Babincová100%100%100%
RNDr. Magdaléna Májeková PhD.100%80%-
Ing. Zuzana Brnoliaková PhD.60%40%-
ISBN: ISBN 978-80-972360-7-6

Antioxidant, antibacterial and anti-biofilm activity of new unnatural gallotannins

Šárka Pospíšilová1,2 , Jana Hricovíniová3 , Alois Čížek , Josef Jampílek4 , Zuzana Hricovíniová5 ,
1 Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute, Palacky University, 783 71 Olomouc, Czech Republic
2 Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, 612 42 Brno, Czech Republic
3 Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, 845 05 Bratislava, Slovak Republic
4 Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic
5 Institute of Chemistry, Slovak Academy of Sciences, 845 38 Bratislava, Slovak Republic
sharka.pospisilova@gmail.com

Tannins represent a large sub-class of polyphenolic compounds ubiquitously present in plants. Naturally occurring gallotannins (GTs) are widely studied due to their multiple prophylactic and therapeutic effects [1]. GTs are basically composed of a central carbohydrate core that is esterified with gallic acid. The most common carbohydrate is d-glucose, although other monosaccharides are present as well. GTs are extensively studied as they exhibit diverse biological activities ranging from antioxidant, antimicrobial, antiviral, anti-inflammatory, immune-modulatory to anticancer effects [2‑4]. As a part of our ongoing studies on the synthesis of biologically important saccharides, as potential drug candidates we have synthesized a new series of GTs and evaluated their biological activities in various experimental systems. Antioxidant, antibacterial and anti-biofilm activities of structurally different GTs derived from branched-chain d-lyxose, d-ribose, l-rhamnose, d-mannose and d-fructose, together with three unnatural analogues [5] of 1,2,3,4,6-penta-O-galloyl-d-glucose (PGG) were examined and mutually compared.

Based on the experimental results obtained in this study it can be concluded that studied compounds are excellent antioxidants and radical-scavenging agents. They exhibited only moderate activity against gram-positive pathogens such as S. aureus and E. faecalis and low antimycobacterial activity. However, they were efficient inhibitors and disruptors of S. aureus biofilms in sub-MIC concentrations and interacted with quorum-sensing system in Chromobacterium violaceum. Moreover, they are non-toxic to eukaryotic cells, thus are promising candidates for further studies in developing new antimicrobial agents.

Thanks: 

This work was financially supported by Slovak grant agency VEGA 2/0022/18 and by the Slovak Research and Development Agency APVV-17-0373.

Sources: 

[1] Chung, K.-T., Wong, T. Y., Wei, C.-I., et al. (1998). Critical Reviews in Food Science and Nutrition, 38, 421.
[2] Cao, Y., Himmeldirk, K.B., Qian, Y. et al. (2014). J. Nat. Med. 68, 465.
[3] Quideau, S., Deffieux, D., Douat-Casassus, C., Pouysegu, L. (2011). Angew. Chem. Int. Ed., 50, 586.
[4] Lin, M.-H., Chang, F.-R., Hua, M.-Y., et al. (2010). Antimicrobial Agents and Chemotherapy, 55, 1021.
[5] Hricovíniová, J., Ševčovičová, A., Hricovíniová, Z. (2020). Toxicol in Vitro 65, 104789.

Discussion

Dobry den, blahozelam k vysledkom, drzim place do dalsej prace. Mohli by ste, prosim Vas specifikovat ako planujete dalej rozvijat biologicku charakteristiku studovanych latok: v akych biologickych systemoch, akymi metódami? Vopred dakujem za odpoved.

Dobrý deň, gratulujem k výsledkom, je to komplexná práca, popisuje viacero efektov látok. Zaujímalo by ma, či máte nejaké hypotézy o mechanizme antibiotickej aktivity, resp. aktivity potláčajúcej tvorbu biofilmov.