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Determination of ploidy level of Hippeastrum sp. by Feulgen staining method

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Determination of ploidy level of Hippeastrum sp. by Feulgen staining method

Przemysław Marciniak 1 Agnieszka Marasek-Ciołakowska 2 Dariusz Sochacki 1

1Warsaw University of Life Sciences, Department of Ornamental Plants, Warsaw, Poland
2The National Institute of Horticultural Research, Skierniewice, Poland
przemyslaw_marciniak@sggw.edu.pl

Hippeastrum sp. (Amaryllidaceae) is an ornamental bulbous plant native to tropical and subtropical regions of America.  It is used worldwide as a cut and potted flower with showy large flowers of various colors. Two genotypes of Hippeastrum ×chmielii (bred by Henryk Chmiel at the Warsaw University of Life Sciences in 1993) and three commercial cultivars of H. hybridum were tested. The aim of this study was to evaluate the applicability of Feulgen staining of metaphase chromosomes to determine the ploidy level of clones 6 and 18 of H. × chmielii and cultivars 'Gervase', 'Rio Negro' and 'Royal Velvet'. After modifying the maceration time of root meristem cells in 1N HCL, the usefulness of the method to determine the ploidy level of the genotypes studied was demonstrated. The base chromosome number of each genotype was x=11, cultivar ‘Rio Negro’ was diploid (2n=22) and the others genotypes were tetraploid (2n=44).

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? Future directions 20.05.2021 08:38
Nice work done, greetings! What intentions do you have in the next step of your project? How would you cultivate the concept of Feulgen staining method in your further research? Thank you in advance for your...Show all comments
Re: ? Future directions 23.05.2021 18:20
Thank you for your question. Adjusting the staining method will allow us to further investigate the ploidy level of the breeding Hippeastrum clones. The results will provide a basic knowledge of the ploidy o...Show all comments
Re: Re: ? Future directions 24.05.2021 06:14
Ok, thank you for the clarifications, good luck in your further work. Best regards, ZB
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