Neuraminidases are hydrolytic enzymes acting on glycosidic linkage of terminal sialic acids in glycoproteins, glycolipids, oligosaccharides, colominic acids and synthetic substrates [1]. Function of these enzymes is essential in pathogenesis of many viral and bacterial infections [2]. Discovery of potent neuraminidase inhibitors, as well as development of high-throughput methods for their activity evaluation is therefore desirable in treatment and prophylaxis of these diseases [3 - 4]. In our work, we have developed a method for evaluation of neuraminidase activity and its inhibitors. In the developed lectin-based method, the lectin Peanut agglutinin (PNA) was used for the determination of galactose moiety exposed after cleavage of sialic acid from immobilized fetuin by neuraminidase from Clostridium perfringens. Described method was performed in microtiter-plates with quantification of desialylation by fluorescent dye. Our next aim is miniaturization of this method to microarray format.
This work was supported by the SRDA grants APVV-14-0294, APVV-15-0111, APVV-16-0088 and VEGA 2/0137/18.
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