The aim of this research was to develop an assay that can detect the cancer biomarker Thomsen-nouvelle (Tn) antigen using ELISA. The study investigated the impact of the size and functional groups of magnetic beads (MBs) on the specific sensitivity of the bioaffinity interaction. We used four different carboxy-modified MBs with varying sizes (250 nm, 500 nm, 1000 nm, and 2800 nm) to evaluate the best MBs for detecting anti-Tn antibodies. We calculated the slopes from calibration curves to determine the detection sensitivities, and assessed the effect of size on the specific sensitivity of detection of anti-Tn antibodies. We obtained a limit of detection (LOD) of (0.31 ± 0.01) ng mL− 1 or (2.10 ± 0.04) pM for analyte detection, and the optimal assay configuration was highly selective and allowed for reliable detection of the analyte in human serum with a recovery index in the range of 102 -104%.
This work was supported by the Slovak Research and Development Agency under the Contract no. APVV-21-0329.
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