Analýza buniek po interferencii s bioaktívnymi látkami asociovanými s myším herpetickým vírusom 68

Analýza buniek po interferencii s bioaktívnymi látkami asociovanými s myším herpetickým vírusom 68

Celkové hodnotenie

Vedecká práca
50%
Prevedenie (dizajn)
40%
Diskusná interakcia
PoužívateľVedecká prácaDizajnDiskusná interakcia
PharmDr. Štefan Husár PhD.60%40%-
Ing. Zuzana Brnoliaková PhD.40%40%-
ISBN: 978-80-972360-4-5

Analýza buniek po interferencii s bioaktívnymi látkami asociovanými s myším herpetickým vírusom 68

Richard Hodoši1 ,
1 Prírodovedecká fakulta Univerzity Komenského, Bratislava, SR
richard.hodosi@gmail.com

Cultivating cells BHK-21 infected with murine herpesvirus with multipicity of infection (MOI) 0,01 at 41°C we obtained biologically active, growth factor-like substances (MHGF).We have purified „growth factor“ associated with MHV-68 (MHGF-68) which is able to change the morphology of cells (normal phenotype on the transformed phenotype, respectively suppress the transformed phenotype of cancer cells). Samples from the culture medium of BHK-21 cells infected with MHV-68 were fractionated using conventional chemical and chromatographic methods, and the resulting fractions were tested for biological activity. In our work we tested the selected fractions of MHGF-68 for antiproliferative effect in vitro by monitoring the effect on cell proliferation, metabolic activity and viability of the cells by xCELLigence analyzer. The obtained experimental results confirmed biological activities of selected fractions of MHGF-68. And this study indicated that the proliferation index of the cells affected with the selected fractions largely interferes with the chemical treatment of the MHGF fractions.

Poďakovanie: 

Táto práca bola realizovaná s finančnou podporou grantov VEGA 1/0061/18 a APVV-0621-12.

Zdroje: 
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